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| Registros recuperados: 76 | |
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| Fuhrmann, Marine; Petton, Bruno; Quillien, Virgile; Faury, Nicole; Morga, Benjamin; Pernet, Fabrice. |
| Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35‰, 21°C). Water from these tanks was used to infect naïve oysters in 2 experimental setups: (1) oysters acclimated or non-acclimated to a salinity of 10, 15, 25 and 35‰ and (2) oysters acclimated to a salinity of 25‰; the... |
| Tipo: Text |
Palavras-chave: Bivalve; Herpesviridae; Infection; Disease transmission; Risk analysis. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00350/46100/45781.pdf |
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| Pernet, Fabrice; Lagarde, Franck; Jeannee, Nicolas; Daigle, Gaetan; Barret, Jean; Le Gall, Patrik; Quere, Claudie; Roque D'Orbcastel, Emmanuelle. |
| Although spatial studies of diseases on land have a long history, far fewer have been made on aquatic diseases. Here, we present the first large-scale, high-resolution spatial and temporal representation of a mass mortality phenomenon cause by the Ostreid herpesvirus (OsHV-1) that has affected oysters (Crassostrea gigas) every year since 2008, in relation to their energetic reserves and the quality of their food. Disease mortality was investigated in healthy oysters deployed at 106 locations in the Thau Mediterranean lagoon before the start of the epizootic in spring 2011. We found that disease mortality of oysters showed strong spatial dependence clearly reflecting the epizootic process of local transmission. Disease initiated inside oyster farms spread... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00181/29249/27663.pdf |
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| Lupo, Coralie; Dutta, Bhagat Lal; Petton, Sebastien; Ezanno, P; Tourbiez, Delphine; Travers, Marie-agnes; Pernet, Fabrice; Bacher, Cedric. |
| Vibrio aestuarianus infection in oyster populations causes massive mortality, resulting in losses for oyster farmers. Such dynamics result from host-pathogen interactions and contagion through water-borne transmission. To assess the spatiotemporal spread of V. aestuarianus infection and associated oyster mortality at a bay scale, we built a mathematical model informed by experimental infection data at 2 temperatures and spatially dependent marine connectivity of oyster farms. We applied the model to a real system and tested the importance of each factor using a number of modelling scenarios. Results suggest that introducing V. aestuarianus in a fully susceptible adult oyster population in the bay would lead to the mortality of all farmed oysters over 6 to... |
| Tipo: Text |
Palavras-chave: Basic reproduction number; Crassostrea gigas; Hydrodynamics; Oyster disease; Sensitivity analysis; Vibrio aestuarianus. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00662/77459/79114.pdf |
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| Couturier, Lydie; Michel, Loic; Amaro, Teresa; Budge, Suzanne M; Da Costa, Elisabete; De Troch, Marleen; Di Dato, Valeria; Fink, Patrick; Giraldo, Carolina; Le Grand, Fabienne; Loaiza, Iván; Mathieu-resuge, Margaux; Nichols, Peter D; Parrish, Christopher C; Sardenne, Fany; Vagner, Marie; Pernet, Fabrice; Soudant, Philippe; Browman, Howard. |
| Determining the lipid content and fatty acid (FA) composition of aquatic organisms has been of major interest in trophic ecology, aquaculture, and nutrition for over half a century. Although protocols for lipid analysis are well-described, their application to aquatic sciences often requires modifications to adapt to field conditions and to sample type. Here, we present the current state of knowledge of methods dedicated to both marine and freshwater lipid analyses, from sampling to data treatment. We review: (i) sample preservation, storage and transport protocols, and their effects on lipids, (ii) lipid extraction, separation of polar and neutral lipids, derivatization, and detection methods, and (iii) available tools for the statistical analysis of FA... |
| Tipo: Text |
Palavras-chave: Aquatic nutrition; Biomarkers; Fatty acids; Marine and freshwater lipids; Methods; Recommended practices. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00651/76279/77252.pdf |
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| Degremont, Lionel; Maurouard, Elise; Nourry, Max; Seugnet, Jean-luc; Bedier, Edouard; Fleury, Elodie; Langlade, Aime; Pernet, Fabrice; Pouvreau, Stephane; Le Souchu, Pierrick; Normand, Julien; D'Amico, Florence; Rumebe, Myriam; Cantin, Christian; Barret, Jean; Le Gall, Patrik; Baud, Jean-pierre; Cochennec-laureau, Nathalie; Gervasoni, Erika; Pelissier, Pablo. |
| Ce rapport présente la comparaison des mortalités de deux lots de naissains de Crassostrea gigas dans plusieurs bassins conchylicoles et déterminer le gain de survie obtenu par la sélection pour des huîtres diploïdes |
| Tipo: Text |
Palavras-chave: Mortalités; Huître creuse; Crassostrea gigas; Sélection génétique; Naissain. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00065/17644/15180.pdf |
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| Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Renault, Tristan; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garriques, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre. |
| Suite aux surmortalités observées au sein des élevages de moules touchant à la fois les animaux adultes et les juvéniles au mois de mars, dans les Pertuis Charentais, la Direction Générale de l’Alimentation (DGAl) a adressé une saisine à Ifremer le 04 avril 2014. Celle-ci fait état d’une demande d’appui scientifique et technique d’Ifremer pour tenter d’apporter des éléments de réponse par rapport à cette mortalité subite et importante. L’Ifremer a proposé un projet d’action permettant l’expertise du phénomène de surmortalité de la moule bleue, selon 3 axes : l’impact environnemental, la pathologie et les caractéristiques physiologiques des moules. Il s’agit de mettre en évidence la présence ou non d’agents infectieux connus ou émergents, d’apporter des... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00229/34022/32387.pdf |
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| Rayssac, Nathalie; Pernet, Fabrice; Lacasse, Olivia; Tremblay, Rejean. |
| Temperature is a major factor contributing to the biogeography of intertidal poikilotherms. The population dynamics of intertidal invertebrates like mussels are strongly dependent on larval supply and larval mortality. Unlike adults, which are resistant to fluctuating environmental conditions, larvae are highly sensitive to the physicochemical conditions of the pelagic zone. The effects of rearing temperature on larval and post-larval performance of 2 coexisting mussels species, Mytilus edulis and M. trossulus, were examined in this study. In the first experiment, larvae of both species were reared separately at constant temperatures of 10, 17, and 24 degrees C from D-larvae to the dissoconch stage. In the second experiment, pediveligers were reared under... |
| Tipo: Text |
Palavras-chave: Thermal adaptation; Bivalve mollusk; Selection; Biogeography; Lipid; Larval ecology. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00019/13028/10159.pdf |
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| Petton, Bruno; Pernet, Fabrice; Robert, Rene; Boudry, Pierre. |
| Since 2008, mass mortalities of 1-yr-old Crassostrea gigas associated with the ostreid herpesvirus OsHV-1 μVar have occurred along all the coasts of France when seawater temperature reaches 16 to 17°C. The present study aimed to characterize the effect of temperature on oyster survival in combination with OsHV-1 DNA quantification by standard real-time PCR and total vibrio population levels in oyster tissues. To examine the effect of seawater temperature on disease transmission and related mortality of oysters, cohabitation experiments were conducted between healthy naïve oysters and oysters previously exposed to field conditions in areas where mortalities were occurring. Oysters initially maintained in controlled conditions (free of mortality and negative... |
| Tipo: Text |
Palavras-chave: Juvenile oyster; Pathology; Temperature; Survival; Vibrios; Ostreid herpesvirus 1. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00148/25891/23912.pdf |
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| Pernet, Fabrice; Browman, Howard I. |
| Aquaculture now produces more seafood than wild capture fisheries and this production is expected to at least double by 2050. Representing almost half of global production, marine aquaculture will contribute to sustainably feeding the growing humanity. However, climate change will undoubtedly challenge the future growth of marine aquaculture. Temperature and sea-level rise, shifts in precipitation, freshening from glacier melt, changing ocean productivity, and circulation patterns, increasing occurrence of extreme climatic events, eutrophication, and ocean acidification are all stressors that will influence marine aquaculture. The objective of this themed article set was to bring together contributions on the broad theme of the potential impacts,... |
| Tipo: Text |
Palavras-chave: Adaptation; Bivalve; Blue economy; Carbon; Climate change; Marine diseases; Mitigation; Ocean acidification; Plasticity; Salmon; Seaweed; Sustainability. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00682/79406/81951.pdf |
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| Redjah, I.; Olivier, Frederic; Tremblay, R.; Myrand, B.; Pernet, Fabrice; Neumeier, U.; Chevarie, L.. |
| Soft-shell clam, Mya arenaria, culture on the east coast of Canada is characterized by high loss following seeding. To evaluate the importance of passive transport due to currents, an experimental flume study was designed. The purpose was to measure the effects of hydrodynamic conditions, substrate, and clam size on dispersal in controlled laboratory condition and to interpret these results in relation to field measurements. Unidirectional currents with gradual increasing velocities (0 to 60 cm s(-1)) were applied to three substrates (muddy sand, medium sand, and coarse sand) in which clams from one of three size classes (10, 15, and 20 mm) had burrowed. We also examined the resulting effects of turbulent kinetic energy on the erosion of medium-grain sand... |
| Tipo: Text |
Palavras-chave: Clam transport; Substratum erosion; Unidirectional currents; Turbulence; ADV; Flume. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00014/12507/9368.pdf |
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| Offret, Clement; Paulino, Sauvann; Gauthier, Olivier; Château, Kevin; Bidault, Adeline; Corporeau, Charlotte; Miner, Philippe; Petton, Bruno; Pernet, Fabrice; Fabioux, Caroline; Paillard, Christine; Blay, Gwenaelle Le. |
| Digestive microbiota provides a wide range of beneficial effects on host physiology and are therefore likely to play a key role in marine intertidal bivalve ability to acclimatize to the intertidal zone. This study investigated the effect of intertidal levels on the digestive bacterial microbiota of oysters Crassostrea gigas and clams Ruditapes philippinarum, two bivalves with different ecological niches. Based on the 16S rRNA region sequencing, digestive glands, seawater and sediments harbored specific bacterial communities, dominated by OTUs assigned to the Mycoplasmatales, Desulfobacterales and Rhodobacterales orders, respectively. Field implantation modified digestive bacterial microbiota of both bivalve species according to their intertidal position.... |
| Tipo: Text |
Palavras-chave: Microbiota; Oysters; Clams; Intertidal zone; Digestive gland; Metabarcoding. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00628/73961/73320.pdf |
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| Petton, Bruno; Destoumieux Garzon, Delphine; Pernet, Fabrice; Toulza, Eve; De Lorgeril, Julien; Degremont, Lionel; Mitta, Guillaume. |
| The Pacific oyster (Crassostreae gigas) has been introduced from Asia to numerous countries around the world during the 20th century. C. gigas is the main oyster species farmed worldwide and represents more than 98% of oyster production. The severity of disease outbreaks that affect C. gigas, which primarily impact juvenile oysters, has increased dramatically since 2008. The most prevalent disease, Pacific oyster mortality syndrome (POMS), has become panzootic and represents a threat to the oyster industry. Recently, major steps towards understanding POMS have been achieved through integrative molecular approaches. These studies demonstrated that infection by Ostreid herpesvirus type 1 µVar (OsHV-1 µvar) is the first critical step in the infectious process... |
| Tipo: Text |
Palavras-chave: Pacific oyster mortality syndrome; Polymicrobial disease; Multifactorial disease; Crassostrea gigas; OsHV-1; Opportunistic bacterial pathogens. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00679/79158/81669.pdf |
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| Delisle, Lizenn; Fuhrmann, Marine; Quere, Claudie; Pauletto, Marianna; Pichereau, Vianney; Pernet, Fabrice; Corporeau, Charlotte. |
| Voltage-dependent anion channel (VDAC) is a key mitochondrial protein. VDAC drives cellular energy metabolism by controlling the influx and efflux of metabolites and ions through the mitochondrial membrane, playing a role in its permeabilization. This protein exerts a pivotal role during the white spot syndrome virus (WSSV) infection in shrimp, through its involvement in a particular metabolism that plays in favor of the virus, the Warburg effect. The Warburg effect corresponds to an atypical metabolic shift toward an aerobic glycolysis that provides energy for rapid cell division and resistance to apoptosis. In the Pacific oyster Crassostrea gigas, the Warburg effect occurs during infection by Ostreid herpesvirus (OsHV-1). At present, the role of VDAC in... |
| Tipo: Text |
Palavras-chave: Voltage-dependent anion channel; Warburg effect; Crassostrea gigas; Ostreid herpes virus. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00420/53129/54142.pdf |
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| Pernet, Fabrice. |
| Le naissain d’huître creuse Crassostrea gigas est touché chaque année depuis 2008 par des mortalités causées par un microvariant de l’ostreid herpesvirus 1 (OsHV-1 μVar). Plusieurs études montrent que ces évènements de mortalité se produisent préférentiellement lorsque la température de l’eau est comprise entre 16°C et 24°C. Par conséquent, une exposition rapide des huîtres infectées par OsHV-1 à des températures supérieures à 24°C pourrait limiter les mortalités causées par le virus. Il est proposé de (1) vérifier l’hypothèse selon laquelle l’exposition à haute température réduit la mortalité des huîtres infectées par OsHV-1, et (2) déterminer si les animaux qui ont survécu suite à l’infection par OsHV-1 et au traitement thermique sont... |
| Tipo: Text |
Palavras-chave: Epidémiologie; Huître; OsHV-1; Risque; Survie; Température; Epidemiology; Oysters; OsHV-1; Risk; Survival; Temperature. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00434/54574/55953.pdf |
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| Registros recuperados: 76 | |
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